= Using atlas data as a spatial reference for a result of a realistic neural network simulation = == Model download == From [[http://www.3dbar.org:8080/getPreview?cafDatasetName=aba2011;structureName=grey,VPL,SSp-bfd1,SSp-bfd2Slash3,SSp-bfd4,SSp-bfd5,SSp-bfd6a,SSp-bfd6b|The Allen Mouse Brain Reference Atlas, 2011 Segmentation]] download the following VRML high quality models: - [[http://www.3dbar.org:8080/getReconstruction?cafDatasetName=aba2011&structureName=grey&qualityPreset=high&outputFormat=vrml|Basic cell groups and regions]], - [[http://www.3dbar.org:8080/getReconstruction?cafDatasetName=aba2011&structureName=SSp-bfd1&qualityPreset=high&outputFormat=vrml|Primary somatosensory area, barrel field, layer 1]], - [[http://www.3dbar.org:8080/getReconstruction?cafDatasetName=aba2011&structureName=SSp-bfd2Slash3&qualityPreset=high&outputFormat=vrml|Primary somatosensory area, barrel field, layer 2/3]], - [[http://www.3dbar.org:8080/getReconstruction?cafDatasetName=aba2011&structureName=SSp-bfd4&qualityPreset=high&outputFormat=vrml|Primary somatosensory area, barrel field, layer 4]], - [[http://www.3dbar.org:8080/getReconstruction?cafDatasetName=aba2011&structureName=SSp-bfd5&qualityPreset=high&outputFormat=vrml|Primary somatosensory area, barrel field, layer 5]], - [[http://www.3dbar.org:8080/getReconstruction?cafDatasetName=aba2011&structureName=SSp-bfd6a&qualityPreset=high&outputFormat=vrml|Primary somatosensory area, barrel field, layer 6a]], - [[http://www.3dbar.org:8080/getReconstruction?cafDatasetName=aba2011&structureName=SSp-bfd6b&qualityPreset=high&outputFormat=vrml|Primary somatosensory area, barrel field, layer 6b]], - [[http://www.3dbar.org:8080/getReconstruction?cafDatasetName=aba2011&structureName=VPL&qualityPreset=high&outputFormat=vrml|Ventral posterolateral nucleus of the thalamus]]. Download also [[attachment:framedata0051.vtk.zip|a model of a of the barrel cortex column]]. Unwrap downloaded archives. == Visualisation == Run [http://www.paraview.org ParaView] software (description for version 4.0.1). Open downloaded *.wrl and *.vtk files. Click the ''Apply'' button (in the tab ''Properties''). [[Screenshot(17,width=400,height=300,description=$description,align=center,format=png)]] In the ''Properties'' tab set ''Styling: Opacity'' to 0.1 for ''scene_grey.wrl'' model. For every ''scene_SSp_bfd*.wrl'' model set ''Styling: Opacity'' to 0.3, then change ''Coloring'' from ''VRMLColor'' to ''Solid Color''. Click ''Coloring: Edit'' and select color to: - Red = 250, Green: 245, Blue: 255 for ''scene_SSp_bfd1.wrl'', - Red = 153, Green: 163, Blue: 255 for ''scene_SSp_bfd2Slash3.wrl'', - Red = 74, Green: 77, Blue: 128 for ''scene_SSp_bfd4.wrl'', - Red = 171, Green: 177, Blue: 255 for ''scene_SSp_bfd5.wrl'', - Red = 145, Green: 149, Blue: 213 for ''scene_SSp_bfd6a.wrl'', - Red = 177, Green: 170, Blue: 255 for ''scene_SSp_bfd6b.wrl''. [[Screenshot(18,width=400,height=300,description=$description,align=center,format=png)]] Click ''Coloring: Edit'' for the ''framedata0051.vtk'' model (ensure that ''Coloring'' is set to ''voltage''). Click the "Gear" icon to edit ''Color Scale''. Set ''Color Space'' to ''CIELAB''; set the left color point to Red = 59, Green = 76 and Blue = 192 and the right to Red = 255, Green = 255 and Blue = 0. Add two color points: at ''Color Scalar Value'' = -40 (Red = 150, Green = 150 and Blue = 0) and at ''Color Scalar Value'' = 18 (Red = 190, Green = 35 and Blue = 21). Click the ''Apply'' button, then close the ''Color Scale Editor'' window. [[Screenshot(19,width=400,height=300,description=$description,align=center,format=png)]] Apply the ''Transform'' filter (''!Filters/Alphabetical/Transform'' from menu) to the ''framedata0051.vtk'' model. In the ''Properties'' tab set the transformation matrix to: ||=Translate =||-3.71011222757522 ||-0.955791920040867 ||-0.855289018330382 || ||=Rotate =||40.2025580192141 ||-63.976105129058 ||76.2999549515704 || ||=Scale =||0.58 ||0.58 ||0.58 || {{{#!comment \\ \\ \\ \\ \\ }}} [[Screenshot(20,width=400,height=300,description=$description,align=center,format=png)]] Click the ''Apply'' button. [[Screenshot(21,width=400,height=300,description=$description,align=center,format=png)]] Choose an appropriate viewport and export the scene or a screenshot if you wish. {{{#!comment \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ \\ }}} [[Screenshot(22,width=400,height=300,description=$description,align=center,format=png)]] [[Screenshot(23,width=400,height=300,description=$description,align=center,format=png)]]